384. ROBUST CULTURE OF PATIENT-DERIVED ESOPHAGEAL ADENOCARCINOMA ORGANOIDS OF SINGLE CELL LINEAGE

Abstract Background Esophageal adenocarcinoma (EAC) has a high mutational rate leading to significant intratumoral heterogeneity. We have previously generated patient-specific organoid models of EAC from endoscopic biopsies and resection specimens. It is unclear whether heterogeneity retained in these models, but we did observe morphologic cultures. In this study, sought generate methods isolate clonal organoids patient-derived Methods Patient derived were resuspended into single cell solution dead cells stained with DAPI. Automatic sorter was used pipette live Matrigel coated 96 well plate. Single per pipetted checked within 24 hours using light microscope. Cells second coat media allowed grow full size organoids. Organoids morphology expanded collected for further analysis. Results established high-throughput method culture deriving cell, allowing lineage grown take up 21 days full-sized distinct morphology. identified 4 morphologies which designated: solid, cystic, budding, grape. Conclusion Isolating the first step studying inter- intra- tumour This allows morphology-transcriptome morphology-drug response correlations.